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Neural Stem Cell Culture Media

RHB-A和RHB-Basal是兩款無血清、成分*確定的培養基，用于人類或小鼠神經干細胞（NS）的定向分化獲取、維持培養、擴增、誘導分化成神經細胞等用途。RHB-A可用來培養擴增貼壁的人或小鼠的神經干細胞。在RHB-A中培養的神經干細胞保持著穩定的神經干細胞分化發育成為成熟的神經細胞的能力 (1-3) 。另外，RHB-A還用來定向分化小鼠胚胎干細胞(mouse ES)至神經前體細胞 (neural precursors) (4-6)。
RHB‐Basal不包含生長因子或是神經細胞相關的添加物。因此，可以RHB‐Basal為基礎，通過添加客戶認為需要的添加物來開發適合客戶的特殊類型細胞的培養基。

■ 產品特點

· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent
human and mouse NS cells
· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be
customized by the addition of supplements

■ 產品應用

· Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
· Maintenance and propagation of adherent mouse and human NS cells
· Differentiation of mouse and human NS cells into functional neurons
· Differentiation of mouse ES cells to neuronal precursors
· Refer to the Data Sheet for additional examples of use

■ 產品詳情

Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).

參考文獻：

1. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
    Nature Biotechnology 21:183-186.
2. Conti L, et al. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell.
    PLoS Biology 3(9):e283.
3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120.
4. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells.
    Biotechnology and Applied Biochemistry 49:105-112.
5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors
    and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403.
6. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS)cells in adherent culture.
    Molecular and Cellular Neuroscience 38:245-258.
7. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are
    suitable for chemical and genetic screens. Cell Stem Cell 4:568-580.
8. Abranches E, et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis
    in the embryo. PLoS ONE 4(7): e6286.
9. Fernandes et al. (2010) Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells.
    Biotechnology and Bioengineering 106: 260–270.
10. Fernandes, T.G., et al. (2010) Different stages of pluripotency determine distinct patterns of proliferation, metabolism,

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TAKARA Cellartis®干細胞研究產品-小鼠ES細胞神經分化培養基 RHB-A&

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