Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
Description
Rabbit polyclonal to Cdk9
Tested applicationsIP more details
Species reactivity
Reacts with: Human Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Immunogen
Synthetic peptide, corresponding to to a region within amino acids 1-50 of Human Cdk9 (NP_001252.1).
Positive control
HeLa whole cell lysate
Properties
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration 100 µg at 1 mg/ml
PurityImmunogen affinity purified
Purification notesab124261 was affinity purified using an epitope specific to Cdk9 immobilized on solid support .
Clonality Polyclonal
IsotypeIgG
Applications
Our Abpromise guarantee covers the use of ab124261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Notes
IP
IP: Use at 2-10 µg/mg of lysate.
Application notesIs unsuitable for or WB.
Target
FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
Tissue specificityUbiquitous.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.